EXPERIMENT

Dated entries from the lab, newest at the bottom. Each entry follows the same layout: Objective, Materials, Procedure and Observations. The results of a day’s plates are usually recorded in the next entry.

ENTRIES

• Oct 02 – Poured IP and MP plates; streaked 8 wastewater samples plus 2 controls.

• Oct 05 – Checked the Oct 02 plates (4 positives per antibiotic); started overnight control cultures.

• Oct 06 – Diluted and spread the library onto IP, MP, Tet and LB plates alongside controls.

• Oct 07 – Checked the Oct 06 plates and stored them at 4°C; started new overnight cultures.

• Oct 08 – Repeated the library screen from Oct 06.

• Oct 09 – Checked the Oct 08 plates and stored them at 4°C.

• Oct 14 – Streaked positive isolates from the Oct 06 and Oct 08 screens onto 40 IP/MP plates.

• Oct 15 – Second streak of wastewater isolates onto 72 plates (62 IP, 10 MP).

• Oct 19 – Made LB agar and poured 52 tetracycline plates.

• Oct 20 – Streaked isolates onto the 52 tetracycline plates.

• Oct 21 – Checked the tetracycline plates; poured 46 ampicillin plates.

• Oct 22 – Streaked 36 isolates from the tetracycline plates onto ampicillin plates.

Entries marked (RECONSTRUCTED) were written up afterwards from my bench notes, rather than on the day.