PROTOCOLS

A sterilization technique that uses pressurized steam at high temperatures to eliminate microorganisms, including bacteria, viruses, and resistant bacterial spores, from laboratory equipment, media, and materials

The preparation of nutrient-rich LB agar plates and liquid LB broth to support bacterial growth and culture maintenance. 

The process of heating media to dissolve ingredients and create a uniform mixture before sterilization or use in microbiological procedures.

The process of gradually bringing frozen biological samples to a suitable temperature for laboratory use while minimizing degradation and preserving sample integrity.

A set of laboratory practices designed to prevent unwanted microorganisms from contaminating samples, equipment, or work areas.

A laboratory technique that uses micropipettes to accurately measure and transfer small volumes of liquid, typically in the microlitre range.

A liquid-handling technique that uses graduated serological pipettes to measure and transfer relatively large volumes of liquid.

The process of pouring sterilized molten agar into sterile Petri dishes and allowing it to solidify to create a suitable surface for microbial growth and culture techniques.

A microbiological technique that spreads a bacterial sample across an agar plate in a pattern to isolate individual colonies.

A method of progressively reducing a sample's concentration through a series of measured dilutions, often used in microbial analysis.

A technique used to distribute a liquid sample evenly across an agar plate to allow bacterial colonies to grow across the surface.

The process of growing microorganisms in a suitable growth medium under controlled conditions over an extended period, typically overnight.